Depending on the measurement conditions, a valid light
source must be selected to get reliable results. Various illuminants
are used for particular applications. Please refer to your procedure instructions
to find the correct illuminant for your analysis.
Typically, measurements in color analysis are performed in 1 cm cuvettes. Generated data is classified as transmittance spectrum.
However, standard cuvettes range from 0.5 cm up to 10 cm. Hence intensities must be corrected in order to contribute the cuvette pathlength.
The relationship is the following:
I (1cm) = I(1/d)
where d = thickness/pathlength of the cell/cuvette